Abstract - 2012 Volume.3 Issue.2

The separation of oxybenzone, octocrylene, octinoxate and avobenzone were carried out on waters C18, 5 μ (250 X 4.6 mm) column. The compounds were eluted using a mobile phase of MeOH : H2O (90:10 % v/v) at a flow rate of 1.0 mL/min and were monitored using UV detector at 330 nm. The retention times were 4.5, 7.6, 11.2 and 12.4 for octocrylene, octinoxate and avobenzone respectively. The reliability and analytical performance of the proposed RP-HPLC procedure were statistically validated with respect to linearity, ranges, system suitability, precision, accuracy, robustness, ruggedness, detection and quantification limits. The linear correlation coefficient at range of 12-28 μg/mL of assay concentration levels was found to be 0.999 for all drugs. Repeatability, inter day precision, intraday precision, ruggedness and robustness expressed as the percentage relative standard deviation (% RSD) were less than 2, accuracy by recovery study of were found to be between 99.0 % and 101.0 %. LOD values were found to be 0.07, 0.28, 2.12 and 2.54 μg/mL for octocrylene, octinoxate and avobenzone respectively. LOQ values were found to be 0.00, 0.00, 0.03 and 0.04 μg/mL for oxybenzone, octocrylene, octinoxate and avobenzone respectively.

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